Molecular Detection of Rabies by SYBR Green Real Time PCR
نویسندگان
چکیده
The present study was envisaged to compare the sensitivity of SYBR Green real time PCR with immunofluorescence PCR for diagnosis of rabies. SYBR Green real time PCR technique was applied on brain samples collected from 39 rabies suspected animals. Sensitivity of SYBR Green technique was compared in accordance with WHO recommended gold standard test viz. Fluorescent Antibody Technique (FAT) applied on brain samples. SYBR Green real time PCR applied on brain samples had successfully confirmed rabies in 16/39 with a Sensitivity of 80% . It was concluded that SYBR Green real time PCR is a useful, specific, sensitive and better molecular approach for diagnosis of rabies from brain of rabid suspected cases and can be used as confirmatory test for rabies diagnosis.
منابع مشابه
Introducing a New SYBR green Real-time PCR for Detection of SARS-CoV2 Virus Genome
Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
متن کاملQuantitative detection of chicken meat routine mislabeling in emulsion type sausages and burgers by SYBR green real time PCR assay
ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...
متن کاملDevelopment of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملCross-Platform Evaluation of Commercial Real-Time SYBR Green RT-PCR Kits for Sensitive and Rapid Detection of European Bat Lyssavirus Type 1
This study evaluates the performance of five two-step SYBR Green RT-qPCR kits and five one-step SYBR Green qRT-PCR kits using real-time PCR assays. Two real-time thermocyclers showing different throughput capacities were used. The analysed performance evaluation criteria included the generation of standard curve, reaction efficiency, analytical sensitivity, intra- and interassay repeatability a...
متن کاملReal-time Sybr Green Pcr for Dog Rabies Diagnosis
The use of a 10-day observation to determine whether a dog is rabid is standard practice. This study was conducted in order to look for evidence of rabies vius in saliva and cerebrospinal fluid (CSF) of suspected live rabid dogs at the time of quarantine by using a SYBR Green real-time RT-PCR based assay for the detection of rabies virus RNA. Saliva and CSF of dogs were collected once on the da...
متن کامل